Biochemical identification and crystal structure of kynurenine formamidase from Drosophila melanogaster

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Biochemical identification and crystal structure of kynurenine formamidase from Drosophila melanogaster.

KFase (kynurenine formamidase), also known as arylformamidase and formylkynurenine formamidase, efficiently catalyses the hydrolysis of NFK (N-formyl-L-kynurenine) to kynurenine. KFase is the second enzyme in the kynurenine pathway of tryptophan metabolism. A number of intermediates formed in the kynurenine pathway are biologically active and implicated in an assortment of medical conditions, i...

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Developmental and genetic studies on kynurenine hydroxylase from Drosophila melanogaster.

The level of kynurenine hydroxylase was measured throughout the development of wild type and the eye color mutants v, cn, st, ltd, cd, kar, w, ca, bri and p(P) of Drosophila melanogaster. In all cases except cn a bimodal distribution of enzyme activity during development was observed. Activity is initially detectable in second instar. A maximum is reached in early third instar. Activity decline...

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Crystal structure of human kynurenine aminotransferase I.

The kynurenine pathway has long been regarded as a valuable target for the treatment of several neurological disorders accompanied by unbalanced levels of metabolites along the catabolic cascade, kynurenic acid among them. The irreversible transamination of kynurenine is the sole source of kynurenic acid, and it is catalyzed by different isoforms of the 5'-pyridoxal phosphate-dependent kynureni...

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Enzymatic studies on the hydroxylation of kynurenine in Drosophila melanogaster.

HE classic pathway for brown pigment synthesis in Drosophila melanogaster, as determined by the accumulation of intermediates, involves the sequence, tryptophan -+ formyl kynurenine + kynurenine -+ 3-hydroxykynurenine -+ brown pigments (for review see ZIEGLER 1961). Two of the enzymes in this pathway. tryptophan pyrrolase (BAGLIONI 1959, 1960; KAUFMAN 1962; MARZLUF 1965a, b) and kynurenine form...

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The Biochemical Characterization of Drosophila melanogaster

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 2012

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj20120416